proteomics and disease biomarkers

1989-08--1994-11   Rutgers University   Ph.D

   

2005.7-current         Beijing Institute of Genomics, Chinese Academy of Sciences

                                    Principal Investigator, Ph.D. Advisor

2004.12-2007.12     Beijing Genomics Institute

                                    Director, GPA (Gene Protein Antibody) Group

2003.11-2004.12     Beijing Institute of Genomics, Chinese Academy of Sciences

                                    Assistant Director, Scientific Affairs

1994.12-1998.7        Worcester Foundation of Biomedical Research, Mass, US

                                    Post-Doctoral Research Fellow

Omics Brush-up
Protein Science and Medical Research
Food Omics

1. Guo J, Xu S, Huang X, Li L, Zhang C, Pan Q, Ren Z, Zhou R, Ren Y, Zi J, Wu L, Stenvang J, Brünner N, Wen B, Liu S. Drug Resistance in Colorectal Cancer Cell Lines is Partially Associated with Aneuploidy Status in Light of Profiling Gene Expression. J Proteome Res. 2016 Nov 4;15(11):4047-59.

2. Xian F, Zi J, Wang Q, Lou X, Sun H, Lin L, Hou G, Rao W, Yin C, Wu L, Li S, Liu S. Peptide Biosynthesis with Stable Isotope Labeling from a Cell-free Expression System for Targeted Proteomics with Absolute Quantification. Mol Cell Proteomics. 2016 Aug;15(8):2819-28.

3. Guo J, Ren Y, Hou G, Wen B, Xian F, Chen Z, Cui P, Xie Y, Zi J, Lin L, Wu S, Li Z,Wu L, Lou X, Liu S. A comprehensive investigation towards the indicative proteins of bladder cancer in urine: from surveying cell secretomes to verifying urine proteins.J Proteome Res. 2016 Jul 1;15(7):2164-77. (co-corresponding)

4. Chen X, Guo D, Zhu Y, Xian F, Liu S,Wu L, Lou X. Nuclear phosphoproteomics analysis reveals that CDK1/2 are involved in EGF-regulated constitutive pre-mRNA splicing in MDA-MB-468 cells. J Proteomics. 2016 Jun 1;141:77-84. (co-corresponding)

5. Xie Y, Chen L, Lv X, Hou G, Wang Y, Jiang C, Zhu H, Xu N,Wu L, Lou X, Liu S. The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression. Oncotarget. 2016 May 31;7(22):32592-606. (co-corresponding)

6. Chen X, Zhu Y, Wang Z, Zhu H, Pan Q, Su S, Dong Y, Li L, Zhang H, Wu L, Lou X, Liu S. mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances. J Proteomics. 2016 Feb 2; 136:13-24. (co-corresponding)

7. Wang Y, Shan Q, Hou G, Zhang J, Bai J, Lv X, Xie Y, Zhu H, Su S, Li Y, Zi J, Lin L, Han W, Zhao X, Wang H, Xu N,Wu L, Lou X, Liu S. Discovery of potential colorectal cancer serum biomarkers through quantitative proteomics on the colonic tissue interstitial fluids from the AOM-DSS mouse model. J Proteomics. 2016 Jan 30; 132:31-40. (co-corresponding)

8. Zhu Y, Chen X, Pan Q, Wang Y, Su S, Jiang C, Li Y, Xu N, Wu L, Lou X, Liu S. A Comprehensive Proteomics Analysis Reveals a Secretory Path- and Status-Dependent Signature of Exosomes Released from Tumor-Associated Macrophages. J Proteome Res. 2015 Oct 2; 14(10):4319-31.

9. Hou G, Lou X, Sun Y, Xu S, Zi J, Wang Q, Zhou B, Han B, Wu L, Zhao X, Lin L, Liu S. Biomarker Discovery and Verification of Esophageal Squamous Cell Carcinoma Using integration of SWATH/MRM. J Proteome Res. 2015 Sep 4; 14(9):3793-803.

10. Hu C, Huang W, Chen H, Song G, Li P, Shan Q, Zhang X, Zhang F, Zhu H, Wu L, Li Y. Autoantibody profiling on human proteome microarray for biomarker discovery in cerebrospinal fluid and sera of neuropsychiatric lupus. PLoS One. 2015 May 8; 10(5):e0126643. (co-corresponding)

11. Zhu J, Liu H, Zhang J, Wang P, Liu S, Liu G, Wu L. Effects of Asn-33 glycosylation on the thermostability of Thermomyces lanuginosus lipase. J Appl Microbiol. 2014 Jul; 117(1):151-9. (corresponding)

12. Liu S, Zhu JH, He LP, Dai J, Lu HB, Wu L, Jin KJ, Yang GZ, Zhu H. Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method. Appl Phys Lett. 2014 Apr 21; 104(16):163701. (co-corresponding)

13. Song G, Hu C, Zhu H, Wang L, Zhang F, Li Y, Wu L. New centromere autoantigens identified in systemic sclerosis using centromere protein microarrays. J Rheumatol. 2013 Apr; 40(4):461-8. (co-corresponding)

14. Song G, Hu C, Zhu H, Li X, Zhao L, Zhou R, Zhang X, Zhang F, Wu L, Li Y. Comparative proteomics study on liver liver mitochondria of primary biliary cirrhosis mouse model. BMC Gastroenterol. 2013 Apr 12; 13:64. (co-corresponding) (co-corresponding)

15. Wang Z, Zhang J, Wang Y, Xing R, Yi C, Zhu H, Chen X, Guo J, Guo W, Li W,Wu L, Liu S, Lu Y. Matrine, a novel autophagy inhibitor, blocks trafficking and the proteolytic activation of lysosomal proteases. Carcinogenesis. 2013 Jan; 34(1):128-38.

16. Shan Q, Lou X, Xiao T, Zhang J, Sun H, Gao Y, Cheng S,Wu L, Xu N, Liu S. A cancer/testis antigen microarray to screen autoantibody biomarkers of non-small cell lung cancer. Cancer Lett. 2013 Jan 1; 328(1):160-7.

17. Hu C, Song G, Huang W, Liu G, Deng C, Zeng H, Wang L, Zhang F, Zhang X, Jeong J, Blackshaw S, Jiang L, Zhu H,Wu L, Li Y. Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics. 2012 Sep; 11(9):669-80. (co-corresponding)

18. Huang W, Hu C, Zeng H, Li P, Guo L, Zeng X, Liu G, Zhang F, Li Y,Wu L. Novel systemic lupus erythematosus autoantigens identified by human protein microarray technology. Biochem Biophys Res Commun. 2012 Feb 10; 418(2):241-6. (co-corresponding)

19. Ru Y, Yin L, Sun H, Yin S, Pan Q, Wei H, Wu L, Liu S. A micropreparation of mitochondria from cells using magnetic beads with immunoaffinity. Anal Biochem. 2012 Feb; 421(1):219-26.

20. Wang Y, Wei H, Pan Q, Wang Z, Xing R, Li W, Zhang J, Ding M, Guo J, Wu L, Lu Y, Liu S. Identification and elimination of heterophilic antibody interference during antibody pair screening. Anal Biochem. 2012 Nov 1; 430(1):1-3.

21. Song Q, Liu G, Hu S, Zhang Y, Tao Y, Han Y, Zeng H, Huang W, Li F, Chen P, Zhu J, Hu C, Zhang S, Li Y, Zhu H,Wu L. Novel autoimmune hepatitis-specific autoantigens identified using protein microarray technology. J Proteome Res. 2010 Jan; 9(1):30-9. (co-corresponding)

22. Hu S., Li Y., Liu G., Song Q, Wang L., Han Y., Zhang Y., Song Y., Yao X., Tao Y., Zeng H., Yang H., Wang J., Zhu H., Chen ZN, andWu L. A Protein Chip approach for high-throughput antigen identification and characterization. Proteomics 2007, 7, 2151-2161. (co-corresponding)

Li Y, Wu L, Hu C, Song G. The autoantigens of primary biliary cirrhosis and their applications. 201210080385.8

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My research interests lie in the area of proteomics, for both technologies and applications. One rather practical interest is biomarker identification for potential clinical applications. By using protein microarray, antibody, and mass spectrometry technologies, we are able to identify autoantibody and protein biomarkers for various diseases. Another area of interest is to understand molecular mechanisms of tumor development, especially cell communications within the tumor microenvironment. On this front, exosomes of tumor-associated macrophage are the current focus. The third and recently developed interest is to study the role of chromosomal binding proteins in chromosome related events, particularly DNA damage repair and cell divisions.

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